| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.116133 |
| Chromosome: | chromosome 14 |
| Location: | 1039924 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g614500 | BTB/POZ domain containing protein; (1 of 25) IPR000210//IPR011333 - BTB/POZ domain // POZ domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATATGGTCACGCTAGCGCTGTGCCCTCGTA |
| Internal bar code: | TCGTCGGGTTCATCCATCATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 652 |
| LEAP-Seq percent confirming: | 99.5818 |
| LEAP-Seq n confirming: | 8572 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACTGCCTGGTGACTTCTG |
| Suggested primer 2: | TCGGTTCATGAGGTAGGGAG |