| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.116136 |
| Chromosome: | chromosome 2 |
| Location: | 6827169 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g119150 | (1 of 8) PF00169 - PH domain (PH) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTTCAAACTCAAAGGCCAGGGGGAGCGG |
| Internal bar code: | ACAGTCTCTGGAATTCAACCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 443 |
| LEAP-Seq percent confirming: | 98.8304 |
| LEAP-Seq n confirming: | 169 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGAGGTACTTCGAGCTCAC |
| Suggested primer 2: | TACACTCATACGCCCTGCTG |