| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.116160 |
| Chromosome: | chromosome 6 |
| Location: | 5198823 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g281800 | (1 of 6) PF09353 - Domain of unknown function (DUF1995) (DUF1995) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTAGGGGCGCACACAATTTCTACGCCCCT |
| Internal bar code: | TGCGTCAGCGCCCCGAGGAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 749 |
| LEAP-Seq percent confirming: | 99.6948 |
| LEAP-Seq n confirming: | 1960 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGTGCCAGTGTAGCGTTGG |
| Suggested primer 2: | ATGGCTTTTTGGGTTCTGTG |