| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.116168 |
| Chromosome: | chromosome 6 |
| Location: | 5358867 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g283550 | CRC2,POC6 | Proteome of centriole protein 6; (1 of 1) K16466 - centrin-3 (CETN3, CDC31) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTATGTCACGTTTCAAGGCCTACATAGTTA |
| Internal bar code: | GCGCTTACGTCGTCGACCGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 804 |
| LEAP-Seq percent confirming: | 99.5787 |
| LEAP-Seq n confirming: | 709 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCTCCACGTCGATCTCTA |
| Suggested primer 2: | CAGCTATGGCATTCGGAAAT |