| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.116194 |
| Chromosome: | chromosome 10 |
| Location: | 5499072 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g459100 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGATGTGCACAATTGACAGCACCCAGACA |
| Internal bar code: | GGGACATCTTTGGCTGACATGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 914 |
| LEAP-Seq percent confirming: | 97.0443 |
| LEAP-Seq n confirming: | 591 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCCCTTTCAAGGTGATGC |
| Suggested primer 2: | TGAACTTGAGCGGTGTCTTG |