| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.116197 |
| Chromosome: | chromosome 5 |
| Location: | 2648448 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g236500 | (1 of 1) 3.5.1.23 - Ceramidase / Acylsphingosine deacylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTCGACCCTTGGCCTCTCCACACACACC |
| Internal bar code: | TATCCGTTCTGTCCGTGGCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 457 |
| LEAP-Seq percent confirming: | 78.7299 |
| LEAP-Seq n confirming: | 5641 |
| LEAP-Seq n nonconfirming: | 1524 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTCCTCTACCTTCCCCTG |
| Suggested primer 2: | CTTGTGCTCTCTCACCTCCC |