Insertion junction: LMJ.RY0402.116202_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.116202
Chromosome:chromosome 7
Location:1536354
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g324150 (1 of 11) PF07173 - Protein of unknown function (DUF1399) (DUF1399) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATCCATGAACGCCACGGGGGATACGCCAC
Internal bar code:CTCAGGTGGCAGGTGATAGGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:650
LEAP-Seq percent confirming:99.7148
LEAP-Seq n confirming:1049
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:ATCCTCATGCGACTATTCCG
Suggested primer 2:ATACGCTGTGACGGACTGTG