| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.116223 |
| Chromosome: | chromosome 17 |
| Location: | 3186696 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g721950 | (1 of 1) PTHR11685:SF119 - E3 UBIQUITIN-PROTEIN LIGASE ARI2-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTGCTACCGACTGGTAGAAGCCAAGGAA |
| Internal bar code: | CTGAAGGGGGGTTTTGCACCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 424 |
| LEAP-Seq percent confirming: | 98.7179 |
| LEAP-Seq n confirming: | 77 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCCTTGCTGTAGCTGTCC |
| Suggested primer 2: | GACGAGGAGAAGGTGTGCTC |