| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.116224 |
| Chromosome: | chromosome 12 |
| Location: | 5633057 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g532200 | AOA2 | Putative alcohol O-acetyltransferase; (1 of 4) 2.3.1.84 - Alcohol O-acetyltransferase / AATASE | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCATCAAGCTGCACCACGACTTCGCCCA |
| Internal bar code: | GACGACTCGGTGCCGCCTATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 753 |
| LEAP-Seq percent confirming: | 87.4875 |
| LEAP-Seq n confirming: | 1748 |
| LEAP-Seq n nonconfirming: | 250 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTTGCTGGGCGTATAAAA |
| Suggested primer 2: | CACTGTTGCCATCTTAGCGA |