| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.116256 |
| Chromosome: | chromosome 6 |
| Location: | 234746 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g250650 | EZY8,DRP4A,DRP4 | Similar to DRP4 (MX-like) class of Dynamin-like GTPases; (1 of 1) IPR000375//IPR001401//IPR020850//IPR022812//IPR023220//IPR027417//IPR030381 - Dynamin central domain // Dynamin, GTPase domain // GTPase effector domain, GED // Dynamin superfamily // Type IV secretion system, VirB5-domain // P-loop containing nucleoside triphosphate hydrolase // Dynamin-type guanine nucleotide-binding (G) domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCAGCCGCTGGGACAGCAGGCTGTTGGC |
| Internal bar code: | AGCTGAACCGCCCGGCCGATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 723 |
| LEAP-Seq percent confirming: | 99.7475 |
| LEAP-Seq n confirming: | 2370 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGTAGAAGTCCAGGCTGC |
| Suggested primer 2: | AATGGCTTCACTTGCTTGCT |