| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.116269 |
| Chromosome: | chromosome 10 |
| Location: | 4559517 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g452400 | CSF1 | Chlamydomonas specific family F protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGGTCTGTCTCTCTCGTCTCAGTCCCGT |
| Internal bar code: | AGTTAGGGACCGGAACCTCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 239 |
| LEAP-Seq percent confirming: | 99.8342 |
| LEAP-Seq n confirming: | 602 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCAGACCTCAGCCCTTCAC |
| Suggested primer 2: | ACAATCTGCTGGCACAACTG |