| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.116270 |
| Chromosome: | chromosome 12 |
| Location: | 4750858 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g523950 | (1 of 1) PTHR21229//PTHR21229:SF2 - LUNG SEVEN TRANSMEMBRANE RECEPTOR // PROTEIN C15H9.5 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGCGGGCTGTACGCAGGCTGACGAGGTA |
| Internal bar code: | ATAGGACCTTCGGGTGAGACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 510 |
| LEAP-Seq percent confirming: | 99.7785 |
| LEAP-Seq n confirming: | 901 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTAGCTGGGGTTCGTGTA |
| Suggested primer 2: | ACTCCAGTCTCCCCGTTTTT |