Insertion junction: LMJ.RY0402.116270_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.116270
Chromosome:chromosome 12
Location:4750870
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g523950 (1 of 1) PTHR21229//PTHR21229:SF2 - LUNG SEVEN TRANSMEMBRANE RECEPTOR // PROTEIN C15H9.5 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTGCCCTGACACGCACCCGCGCCATCCCC
Internal bar code:ACGTACGGGTACAAGGGGCTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:621
LEAP-Seq percent confirming:99.8035
LEAP-Seq n confirming:508
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ACTCCAGTCTCCCCGTTTTT
Suggested primer 2:AGCTAGCTGGGGTTCGTGTA