| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.116304 |
| Chromosome: | chromosome 10 |
| Location: | 5608039 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g460100 | (1 of 24) IPR001680//IPR017986//IPR020472 - WD40 repeat // WD40-repeat-containing domain // G-protein beta WD-40 repeat | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTACCAGGCGGGGCATGCGCTCCCCCTGCA |
| Internal bar code: | AAGAGTGGTGGGACGGAATTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 805 |
| LEAP-Seq percent confirming: | 99.1361 |
| LEAP-Seq n confirming: | 1836 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGACGTTAGGTGTAGCCG |
| Suggested primer 2: | GGTACCTGCTTCAAGGGTTG |