Insertion junction: LMJ.RY0402.116340_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.116340
Chromosome:chromosome 17
Location:3190967
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g722000 MFT30,SOC1 Major facilitator superfamily transporter; (1 of 1) K08214 - MFS transporter, OCT family, solute carrier family 22 (organic cation transporter), member 18 (SLC22A18) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGAGAAAGCGAGTTGAGTGTCCGCAAGGAT
Internal bar code:TCCCAAAACCATGCCATGGATT

Confirmation - LEAP-Seq

LEAP-Seq distance:814
LEAP-Seq percent confirming:99.8497
LEAP-Seq n confirming:1993
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:AACAGGGAGGCAGTACATGG
Suggested primer 2:GTATACGCCGACCACGAAGT