| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.116342 |
| Chromosome: | chromosome 13 |
| Location: | 554208 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g565321 | (1 of 2) 4.3.2.2 - Adenylosuccinate lyase / Succino AMP-lyase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTTGAGGCTGGGCATGTGGGCAAGTGGG |
| Internal bar code: | ATGTTCCCAACCCCTATTGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 453 |
| LEAP-Seq percent confirming: | 98.9021 |
| LEAP-Seq n confirming: | 3423 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACCGACCTCACATTCGTT |
| Suggested primer 2: | CTGTAGCCAATTGCTGCTGA |