| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.116351 |
| Chromosome: | chromosome 2 |
| Location: | 1948895 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g087950 | TSM1 | Methionyl-tRNA synthetase; (1 of 1) PTHR11946:SF86 - METHIONINE--TRNA LIGASE, CYTOPLASMIC | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATGAATGCAGCCCCCTGCCTCCCTGCTC |
| Internal bar code: | GTCGATCGCCAAACTCGCGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 617 |
| LEAP-Seq percent confirming: | 98.8581 |
| LEAP-Seq n confirming: | 2251 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAATGAGGGAGTGCAAGTGG |
| Suggested primer 2: | CGTTGTGTTATCGATGTCCG |