| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.116417 |
| Chromosome: | chromosome 14 |
| Location: | 2055437 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g622000 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTGGTGCCAAACGCCACCGAGAGGACCA |
| Internal bar code: | AGTATGGCCCGGTCGAACATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 244 |
| LEAP-Seq percent confirming: | 56.7495 |
| LEAP-Seq n confirming: | 1051 |
| LEAP-Seq n nonconfirming: | 801 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCACATGAAGGGAGAGGT |
| Suggested primer 2: | TGTATCTGTGCCTGTGCCTG |