| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.116446 |
| Chromosome: | chromosome 10 |
| Location: | 2475596 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g436050 | FSD1 | Fe superoxide dismutase; (1 of 1) PTHR11404//PTHR11404:SF8 - SUPEROXIDE DISMUTASE 2 // SUPEROXIDE DISMUTASE [FE] 1, CHLOROPLASTIC | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTAGGCAAGGCCCGGTAGTGGTTCAGTCG |
| Internal bar code: | GTCCTCACTGCAGCCGAGCTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 465 |
| LEAP-Seq percent confirming: | 99.7055 |
| LEAP-Seq n confirming: | 11171 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTGAGGACACACCAATGC |
| Suggested primer 2: | CAAGGAGGAGTTCAAGCAGG |