Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.116476 |
Chromosome: | chromosome 12 |
Location: | 8785869 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g545401 | 5'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGGACGAGCCGACTCAAGAGGGGTTTTG |
Internal bar code: | GCGAGGAGGGCCCGTGGTAGCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 662 |
LEAP-Seq percent confirming: | 99.927 |
LEAP-Seq n confirming: | 1369 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCATGCACCTTGAATGAATG |
Suggested primer 2: | CACCATAAAGACACGCATGG |