| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.116487 |
| Chromosome: | chromosome 8 |
| Location: | 1409638 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g364200 | (1 of 70) PF07282 - Putative transposase DNA-binding domain (OrfB_Zn_ribbon) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTTTAATCGCCTGCCGCGGCGCCCTTGG |
| Internal bar code: | CTCTCACCTTTATCCTAGGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 541 |
| LEAP-Seq percent confirming: | 97.2477 |
| LEAP-Seq n confirming: | 9752 |
| LEAP-Seq n nonconfirming: | 276 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATTGGACTGAACCTGTGCC |
| Suggested primer 2: | CTTCGCTAAACAAAGCCCAG |