| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.116506 |
| Chromosome: | chromosome 16 |
| Location: | 2451945 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g660601 | (1 of 1) IPR000772//IPR003609 - Ricin B, lectin domain // PAN/Apple domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTACATGCGAAGCTAGTTCGTAGGCATAC |
| Internal bar code: | GGCTAATTTCTGGCGACTGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 776 |
| LEAP-Seq percent confirming: | 98.8372 |
| LEAP-Seq n confirming: | 85 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAACAGCGCACAAGATCAA |
| Suggested primer 2: | ATCTGCTACTGCCACCTGCT |