| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.116542 |
| Chromosome: | chromosome 10 |
| Location: | 4178617 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g449800 | (1 of 1) PF04657 - Putative inner membrane exporter, YdcZ (DMT_YdcZ) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACGCATTCGTTGGCACCACGTGGCATTA |
| Internal bar code: | GATCCTATCCAACTAGGGAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 598 |
| LEAP-Seq percent confirming: | 99.8144 |
| LEAP-Seq n confirming: | 4840 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAGAAAGTAGCGTTCCCA |
| Suggested primer 2: | CTGCTGTGGACAAATCGCTA |