Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.116547 |
Chromosome: | chromosome 13 |
Location: | 4821293 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
intergenic |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCAGCAACGCCGTGTTGCTAACAGTTAA |
Internal bar code: | GTGGGAGCTGCCCTCGATTTTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 570 |
LEAP-Seq percent confirming: | 79.0645 |
LEAP-Seq n confirming: | 2451 |
LEAP-Seq n nonconfirming: | 649 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAGTGACTTGAAGTGCGGAA |
Suggested primer 2: | CGACATAATGCACATACCGC |