| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.116587 |
| Chromosome: | chromosome 13 |
| Location: | 4218859 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g592550 | (1 of 2) PF13661 - 2OG-Fe(II) oxygenase superfamily (2OG-FeII_Oxy_4) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCAGTAGCACTGCCTCCGGCCTCTGCTA |
| Internal bar code: | GTGCGGACCGGATGGCGCGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 848 |
| LEAP-Seq percent confirming: | 80.815 |
| LEAP-Seq n confirming: | 9023 |
| LEAP-Seq n nonconfirming: | 2142 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGCAATTTTGAGCAGCCT |
| Suggested primer 2: | CAGCAGTAGCAGGTGAGCAG |