| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.116616 |
| Chromosome: | chromosome 17 |
| Location: | 631589 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g700300 | CGL22 | cdc48-like protein; (1 of 3) PF00004//PF07724 - ATPase family associated with various cellular activities (AAA) (AAA) // AAA domain (Cdc48 subfamily) (AAA_2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCTGCAGCGCCCATCGCAGCGGCAAGCA |
| Internal bar code: | GTCTATTTTGAATTGACTCACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 262 |
| LEAP-Seq percent confirming: | 23.0769 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 100 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTAGGGTGTCAGTGCTTGC |
| Suggested primer 2: | TAGCACTGTGCAGCTGTGTG |