| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.116618 |
| Chromosome: | chromosome 12 |
| Location: | 5635085 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g532250 | (1 of 12) IPR000595//IPR018490 - Cyclic nucleotide-binding domain // Cyclic nucleotide-binding-like | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAAACAAAGCTTGGAACTGCGGACGGGG |
| Internal bar code: | GTTTACATTATATGTGTTGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 132 |
| LEAP-Seq percent confirming: | 99.3139 |
| LEAP-Seq n confirming: | 579 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGGTGAACTTGAAAGGGTA |
| Suggested primer 2: | ATGCGTACACAGCACTCAGC |