| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.116631 |
| Chromosome: | chromosome 13 |
| Location: | 2452496 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g579901 | DEG6 | (1 of 2) PTHR22939:SF67 - PROTEASE DO-LIKE 9; Deg protease | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGGCGGTGATCAGGGAAGGGGGTAAGGC |
| Internal bar code: | CGTGGGTCTTGATGCGGTGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 717 |
| LEAP-Seq percent confirming: | 98.4107 |
| LEAP-Seq n confirming: | 3839 |
| LEAP-Seq n nonconfirming: | 62 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCATAGGGAGGAGGCTGGT |
| Suggested primer 2: | TACAACCAGAAGGTGGGGAG |