| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.116640 |
| Chromosome: | chromosome 12 |
| Location: | 5264923 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g528450 | ASO1,LASPO1 | L-aspartate oxidase; (1 of 1) 1.4.3.16 - L-aspartate oxidase / LASPO | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCGAGCTTCCATCAGCCACAGCTTCGCA |
| Internal bar code: | AAACACATAAGCAGTCGGACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 983 |
| LEAP-Seq percent confirming: | 99.7129 |
| LEAP-Seq n confirming: | 2778 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGATGTAAACGATGCCAC |
| Suggested primer 2: | GTTGGCTGAGAAAGGAGCAC |