Insertion junction: LMJ.RY0402.116662_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.116662
Chromosome:chromosome 7
Location:2934656
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g332750 PSL2 Protein of unknown function; (1 of 19) IPR000225//IPR016024 - Armadillo // Armadillo-type fold CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGTCCAGAGGCTCCGCGGGCGCACTGCGC
Internal bar code:CGGAACCCGTCTACCCGGTGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:624
LEAP-Seq percent confirming:99.1687
LEAP-Seq n confirming:14196
LEAP-Seq n nonconfirming:119
LEAP-Seq n unique pos:81

Suggested primers for confirmation by PCR

Suggested primer 1:CGGTCTCATTTGATCGTGTG
Suggested primer 2:AACAACACCCTGGTACTCGC