| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.116681 |
| Chromosome: | chromosome 9 |
| Location: | 4445771 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g395621 | DIV122 | (1 of 1) PTHR10933:SF9 - LD07294P | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACTGCTGCTTTGACTCGGGGCCGATGCT |
| Internal bar code: | GAGATGCCGTGGATCAAGGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 336 |
| LEAP-Seq percent confirming: | 24.1909 |
| LEAP-Seq n confirming: | 583 |
| LEAP-Seq n nonconfirming: | 1827 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATATTTAGGGGCCGAGAGC |
| Suggested primer 2: | GACTTTTGCGCCCTAGTGAC |