| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.116689 |
| Chromosome: | chromosome 15 |
| Location: | 894628 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g639400 | (1 of 2) 2.6.99.4 - Transferred entry: 2.3.1.234 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTGGAGGAGGACTTGCAGTTTGCGCTAC |
| Internal bar code: | GTCGTGTCCGTGTGCCAGCTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 527 |
| LEAP-Seq percent confirming: | 94.033 |
| LEAP-Seq n confirming: | 2679 |
| LEAP-Seq n nonconfirming: | 170 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGCAATAACAAAGCAGTGA |
| Suggested primer 2: | ATTGGCATTGGCATAAGAGG |