| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.116699 |
| Chromosome: | chromosome_17 |
| Location: | 3425225 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g724400 | FAP257 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTTCTTCAATTTTCATGTCATGGCCCACTG |
| Internal bar code: | AATGAGGCTCCGTCGGGAATAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 951 |
| LEAP-Seq percent confirming: | 99.8214 |
| LEAP-Seq n confirming: | 559 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGAAAGCCCTGAACACTC |
| Suggested primer 2: | GTAATCTCCTGAATCCGGCA |