| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.116720 |
| Chromosome: | chromosome 3 |
| Location: | 2510963 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g159851 | (1 of 2) IPR001623//IPR002110//IPR020683 - DnaJ domain // Ankyrin repeat // Ankyrin repeat-containing domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCCTTGAGTGCTTTATAGATGGGATACG |
| Internal bar code: | GTCCACGCCTTTCACCGACGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1173 |
| LEAP-Seq percent confirming: | 98.8837 |
| LEAP-Seq n confirming: | 1683 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGGGAGTACCGGGAGACT |
| Suggested primer 2: | CAATATTGGGACATCCCCTG |