Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.116733 |
Chromosome: | chromosome_16 |
Location: | 2815171 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g663200 | FAP295 | Flagellar Associated Protein, cyclic nucleotide dependent protei | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGAAGACCTGGCGGTCCAGGGCCCACACCA |
Internal bar code: | TAGCGGCCCAGATGACTAGCCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 830 |
LEAP-Seq percent confirming: | 92.0096 |
LEAP-Seq n confirming: | 3063 |
LEAP-Seq n nonconfirming: | 266 |
LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAACCACCTGGTTCGACTTT |
Suggested primer 2: | CTGTAATGTGCGTGGATTGG |