Insertion junction: LMJ.RY0402.116738_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.116738
Chromosome:chromosome 4
Location:2439822
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g221500 SMM13 S-adenosyl-L-methionine-dependent methyltransferase; (1 of 228) IPR029063 - S-adenosyl-L-methionine-dependent methyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAAGATAGGGACACACTCAGCCCAATGACT
Internal bar code:TCGGGGCGCTTCCGCTGGGCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:7
LEAP-Seq percent confirming:99.6747
LEAP-Seq n confirming:2758
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CGCCTACCTCGCTACACTTC
Suggested primer 2:TCCTCCCCATCTGTTCTTTG