| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.116742 |
| Chromosome: | chromosome_17 |
| Location: | 4492980 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g732500 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCCGCAGCAGCGGCCGCTGCCGCGCTGAAC |
| Internal bar code: | AGCACAATCGCGGAAGGACTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 358 |
| LEAP-Seq percent confirming: | 99.0654 |
| LEAP-Seq n confirming: | 106 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTCCTGCATGTCTTGAAC |
| Suggested primer 2: | CAAAGGCAGCAGCTGTAGTG |