Insertion junction: LMJ.RY0402.116766_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.116766
Chromosome:chromosome 3
Location:1024422
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g148400 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATCTGCTACCGGCGACAACTCAGGGGCTC
Internal bar code:GATGGCGCCTGACCGCTATAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:832
LEAP-Seq percent confirming:99.8845
LEAP-Seq n confirming:865
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:GTCCTTCTTCTGCCAACAGC
Suggested primer 2:CACAACACCCACCATAGCTG