Insertion junction: LMJ.RY0402.116773_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.116773
Chromosome:chromosome 2
Location:4968173
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g103950 MIA1,FAP100 (1 of 2) PF13863 - Domain of unknown function (DUF4200) (DUF4200); MIA1 subunit of the modifier of inner arms (MIA) complex CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTTGCAACAAGAAGATTTCGCGCTTCCTG
Internal bar code:AGGGTGCGTTGGCGTTGAACAG

Confirmation - LEAP-Seq

LEAP-Seq distance:873
LEAP-Seq percent confirming:99.5536
LEAP-Seq n confirming:223
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TGCACTAGCCGTCTTCAATG
Suggested primer 2:GCCTACGAGACGATGCTAGG