| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.116782 |
| Chromosome: | chromosome 9 |
| Location: | 2866665 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388500 | (1 of 13) PF09273 - Rubisco LSMT substrate-binding (Rubis-subs-bind) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTGAGCTAGTTGAGAACCGTTAAGGGAG |
| Internal bar code: | CATGGAGCAGGGGTTTCAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 970 |
| LEAP-Seq percent confirming: | 99.3274 |
| LEAP-Seq n confirming: | 4873 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTGCAGGTGTTCTCCTC |
| Suggested primer 2: | GGTTGCCATGTACAGTCGTG |