| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.116791 |
| Chromosome: | chromosome 11 |
| Location: | 1680407 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467778 | (1 of 1) K00761 - uracil phosphoribosyltransferase (upp, UPRT) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGGAGGCTGTCGGGAAAACGCAACGCTC |
| Internal bar code: | GTATAGTAGTGGACATTGCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1148 |
| LEAP-Seq percent confirming: | 99.4752 |
| LEAP-Seq n confirming: | 2464 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTGAGAACTCGCAACCTG |
| Suggested primer 2: | TAGCTTCGAGTGTGTCGTGG |