| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.116824 |
| Chromosome: | chromosome 14 |
| Location: | 295659 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g609750 | (1 of 6) PF03351 - DOMON domain (DOMON) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCCCCAACACACCGCTTCACATGCCCCT |
| Internal bar code: | GAACTAGAAGTGGCACCCATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 850 |
| LEAP-Seq percent confirming: | 99.7837 |
| LEAP-Seq n confirming: | 2307 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAATACTCGGTACCCTGGC |
| Suggested primer 2: | CACCACTGCCTGCTGATAGA |