Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.116856 |
Chromosome: | chromosome 10 |
Location: | 5562199 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
intergenic |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGCCGCTCCAGCACCGCCGCCCGCACTG |
Internal bar code: | GTTGGTCGTGGTGAAGAAGCTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 292 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 1517 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCTGGCTGTCATGTTGCTTA |
Suggested primer 2: | GATGACACGCTGATGAATGG |