Insertion junction: LMJ.RY0402.116861_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.116861
Chromosome:chromosome 5
Location:340771
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre05.g233303 (1 of 1) K11718 - UDP-glucose:glycoprotein glucosyltransferase (HUGT) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCATCACGCATGGACGGTTCACCCCCCCC
Internal bar code:GACAGGAGTGCAGAGTTGCCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:83
LEAP-Seq percent confirming:89.2308
LEAP-Seq n confirming:1044
LEAP-Seq n nonconfirming:126
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:TACACACATGGCTTACCCGA
Suggested primer 2:AAGGAGGGGATTCCTTCTGA