Insertion junction: LMJ.RY0402.116882_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.116882
Chromosome:chromosome 1
Location:2857541
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre01.g017200 FKB24,FKB99 Peptidyl-prolyl cis-trans isomerase, FKBP-type; (1 of 2) PTHR10516//PTHR10516:SF290 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE // PEPTIDYL-PROLYL CIS-TRANS ISOMERASE intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTAAGAGGCAGTTGTTTTACACTACATGG
Internal bar code:ACATCTGCTGTATTTGCTACGT

Confirmation - LEAP-Seq

LEAP-Seq distance:177
LEAP-Seq percent confirming:97.7064
LEAP-Seq n confirming:213
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TGTTGAGGAGGCTCTGTCCT
Suggested primer 2:CCACACAGCTGTCAAGGAGA