| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.116891 |
| Chromosome: | chromosome 12 |
| Location: | 5409039 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g530000 | (1 of 11) 2.7.11.24 - Mitogen-activated protein kinase / Stress-activated protein kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCAGTGTACAGTAGCAGGGGTAGCTTTG |
| Internal bar code: | GAGTGGTGGGCGAGGCACGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 395 |
| LEAP-Seq percent confirming: | 99.8899 |
| LEAP-Seq n confirming: | 907 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGGCATTTGTGGAGAAGT |
| Suggested primer 2: | AACAGGGACACGGATGCTAC |