| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.116901 |
| Chromosome: | chromosome 6 |
| Location: | 6310914 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g291750 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGAAGGGACCGTCCAATGAGGGACCATC |
| Internal bar code: | CCTGCCTGTAGGCTATACGTCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 877 |
| LEAP-Seq percent confirming: | 99.3879 |
| LEAP-Seq n confirming: | 2111 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGAAGGATTTGTTGGGGAA |
| Suggested primer 2: | CTTGCCTAAGGTTGTCGAGC |