| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.116903 |
| Chromosome: | chromosome 6 |
| Location: | 3506094 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278105 | (1 of 24) IPR006311 - Twin-arginine translocation pathway, signal sequence | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCAGACGCAGAAAACCCTGAACGCAGGA |
| Internal bar code: | CAACGGCAATACCGGGCTGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 367 |
| LEAP-Seq percent confirming: | 99.8941 |
| LEAP-Seq n confirming: | 2831 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAGGACCTCGGATCAAAC |
| Suggested primer 2: | CGCGGGAAATGTTAAAAATG |