| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.116924 |
| Chromosome: | chromosome 6 |
| Location: | 3021535 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g273800 | (1 of 1) PTHR23115//PTHR23115:SF180 - TRANSLATION FACTOR // GTP-BINDING PROTEIN 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGCGTCACTGACCAAGTAGGCAAGTACT |
| Internal bar code: | ATGCCGAAATGCATTCTTCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 308 |
| LEAP-Seq percent confirming: | 95.8549 |
| LEAP-Seq n confirming: | 185 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGAATAGGGCTACAATGC |
| Suggested primer 2: | GCATATTTGTCCCACAGGCT |