Insertion junction: LMJ.RY0402.116924_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.116924
Chromosome:chromosome 11
Location:1294999
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g467710 VSP1 Hydroxyproline-rich cell wall glycoprotein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGTTACTACCCGAGCTGGAGCCCTAAGAC
Internal bar code:AAGGACCGCGCGCTCCTGGCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:816
LEAP-Seq percent confirming:98.4418
LEAP-Seq n confirming:1769
LEAP-Seq n nonconfirming:28
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CTCGGCTACTCACTGGGAAC
Suggested primer 2:CAGCATTATGCCTACGAGCA